WebSHAO Yina, WANG Jiahao, ZHANG Yi, and LI Chenghua, 1) State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Ningbo University, Ningbo 315211, China WebIt was confirmed that a recombinant plasmid derived from pHIG5-3 by insertion of the BamHI linker at the PvuII site had been ... 5 mM dithiothreitol, 0.1 mM EDTA and 1 mM ATP, 30 units of T4 polynucleotide kinase (Takara Shuzo) were added, and phosphorylation reaction was carried out at 37° C. for 60 minutes. Then, 0.4 μg of the pILAl ...

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WebM13pUC-rev. Bacterial Resistance: Ampicillin. Notes: Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. … WebThe PvuII-HindIII fragment was modified with T4 DNA polymerase at its termini and thereto was linked a phosphorylated XbaI linker with T4 DNA ligase. After digesting the ligate with restriction enzyme XbaI (Takara #1093A), the cleaved fragments were subjected to 6 % of acrylamide gel electrophoresis to prepare a XbaI-XbaI fragment of about 350 bp. lapa lapa menu

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WebJun 6, 2016 · MCRS1 is involved in multiple cellular activities, including mitotic spindle assembly, mTOR signaling and tumorigenesis. Although MCRS1 has been reported to bind to the dynein regulator NDE1, a ... Web根据 Genome Walker TM Universal Kit (Clontech) 实验手册, 分别用限制性内切酶 DraI、EcoRV、PvuII 体系中完全酶切约2.5 基因组DNA, 建立连接特异接头的酶切 DNA 库。采用巢式 PCR 方法, 作模板,建立 个平行的50 PCR反应体系。设计位于 Prkrip1 末端的一对引 … WebPvuII does not cut CAGm4CTG, while cutting at CAGm5CTG occurs at a significantly reduced rate. For enzymes that cannot be heat-inactivated, we recommend using a … lapa lapa menu bebidas