Crispr editing of elite inbred parent lines
WebMay 11, 2024 · Another HI-editing technology (HI-Edit) has been developed to directly edit elite inbred lines of diverse monocot and dicot species by a single cross. In MALT … WebApr 11, 2024 · To obtain new alleles of the S5 locus, two adjacent targets sites of ORF4 located in exon 1 were selected and constructed into the CRISPR/Cas9 gene editing vectors, and transformed into the inbred HJX74-SSSL 10-06 (Figure 3A). Six T 0 generation plants were amplified by PCR-specific primers, and positive plants were screened.
Crispr editing of elite inbred parent lines
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WebMay 1, 2024 · We created waxy corn hybrids by CRISPR–Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than … WebNov 14, 2024 · Backcross breeding is an effective method to transfer one or a few genes controlling a specific trait from one line into a second—usually elite—breeding line. The parent with the desired trait, called the donor parent, provides the desired trait and may not perform as well as an elite variety in other areas. The elite line, called the ...
Webinbred lines commonly used in Chinese maize breeding programs provided the basis for association mapping in this study [38]. There is, however, little GWAS information for SNP markers associated with dwarf loci in panels of elite inbred Chinese maize lines and germplasm, particularly for the widely used inbred line Shen5003. WebFeb 4, 2024 · (A) Combining haploid induction and gene editing (HI-Edit) in maize. A WT elite maize inbred line is pollinated using a haploid inducer line that contains a …
WebThus far, this tool has been almost exclusively used for gene knockouts and deletions, with a few examples of gene edits and targeted gene insertions. Here, we demonstrate the … WebDec 2, 2024 · CRISPR has been used to experiment with gene-edited mosquitos to reduce the spread of malaria, for engineering agriculture to withstand climate change, and in human clinical trials to treat a range of diseases, from cancer to transthyretin amyloidosis, a rare protein disorder that devastates nerves and organs.
WebFeb 16, 2024 · When Sandy was born in 1967, people with CF had an average life expectancy of 15, but during 1970–1990, life expectancy doubled due to new medical …
WebFeb 24, 2024 · Humanity first appears as something more than an object of gene editing in the last line of the book: “CRISPR is moving faster than society can keep up. To where is up to all of us.” Yet most ... styley plantesWebSep 5, 2024 · This clean event with the precise CRISPR-Cas9 edit was used for further validation. Plants hemizygous for the edit were crossed as males to PHFFB, an inbred line containing CMS-C cytoplasm and RF4-Y187, to create an isogenic population segregating 1:1 for the alleles Rf4-Y187/ Rf4-Y187 and Rf4-F187/ Rf4-Y187. This isogenic population … paige snyder lancaster pa mylifeWebOct 23, 2024 · Genome-editing tools including meganucleases, zinc finger nucleases, transcription activator-like effector nucleases and clustered regularly interspaced short palindromic repeats (CRISPR) system have been applied to improve the quality of staple, oilseed, and horticultural crops with great accuracy and efficiency compared to … stylezbypleasurepWebMar 4, 2024 · To overcome these problems, we devised haploid induction (HI) editing technology (HI-Edit, Fig. 1a), which enables direct editing of elite inbred lines by a … Full Size Table - One-step genome editing of elite crop germplasm during haploid … Supplementary Figure 1 The V1 Recessive Pale Leaf Phenotype is Used to Identify … Supplementary Figure 3 Sequencing Results From Five Colonies Subcloned … Supplementary Figure 4 Family Tree of The Vlhp Genes in Chinese Spring Wheat, … Supplementary Figure 2 Flow Cytometry Results From Seedling Leaf of Control … Supplementary Figure 9 Ploidy Diagrams of Diploid Wheat and Jswer30a22 Wheat … Supplementary Figure 10 Expression of Cas9 Under Different Promoters - One … Supplementary Figure 7 PCR Colony Sequencing of Gl1 Edited Haploids - … Supplementary Figure 6 Sequencing of The Gw2-2 and Vlhp2 Target Sites in Edited … Supplementary Figure 11 Negative PCR Assays for Putatively Edited Wheat … paiges mum love islandWebNov 9, 2024 · Meanwhile, new applications of HI systems have been reported, including HI-Edit 3, which introduces genome-editing components to elite varieties via an HI line. This process produces transgene ... style zerolengthpredicateWebIn the few short years since Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated proteins (Cas) were first described, their use in editing the mouse genome has … paiges music phone numberpaiges music shop